In this study, we introduce a new method for identifying the effects of genetic variations on cancer and immune disorders by using CRISPR-based base editing to screen INFg and related genes. By making many mutations in a gene at once (tilling), we can gain a detailed understanding of how different parts of the gene (e.g. funcitonal domains and protein interfaces) contribute to its function and cellular phenotype. This approach allows us to quickly and efficiently determine the functions of genes within important biological pathways.