Crispy: CRISPR-Cas9 Copy Number Bias

We developed a computational approach to correct copy-number driven deleterious bias induced by Cas9 double-strand breaks which leads to increased false discovery rates when targeting amplified regions and reported that this is strongly determined by structural rearrangements.

Crispy Python Package

Reference

Gonçalves, Emanuel, Fiona M. Behan, Sandra Louzada, Damien Arnol, Euan A. Stronach, Fengtang Yang, Kosuke Yusa, Oliver Stegle, Francesco Iorio, and Mathew J. Garnett. 2019. “Structural Rearrangements Generate Cell-Specific, Gene-Independent CRISPR-Cas9 Loss of Fitness Effects.” Genome Biology 20 (1): 27.